New Features
These are the latest features added to ModFit LT 3.1: Version
3.1 Service Pack 3
The program now supports high-resolution, digital data up to
32-bits. This includes support for 18-bit FCS data files exported by
BD's Diva software.
A "Convert" button has been added to the listmode Choose
Parameter dialog. This button displays the Edit Properties for Log
Conversion dialog that allows linear parameters to be converted into
log parameters. Use this option when reading Diva-generated files
that were stored in linear, FCS 3.0 format. The dialog displays a
list of all parameters in the file, allowing the user to select
parameters to convert to log for display and analysis. It also
provides an option to convert to 4-decades or the "computed" decades
for the native, linear resolution.
The program now supports FCS files containing more than one
dataset. When a multi-dataset file is opened, the program displays
the Choose Dataset dialog to allow the user to select the dataset
which should be used for analysis. The selection remains in effect
until it is invalid or the user makes a new choice. The dialog can
be redisplayed by enabling the "Multi Dataset Prompt" property in
the File Batch Settings dialog.
When the program is configured to base ploidy determination on an
external reference or an internal standard, several new results are
reported. The results will display a "Reference Diploid" value that
shows the location where the Diploid G1 is expected to be. In
addition, each cell cycle shows a "DI based on reference:"
statistic, which is computed as the G1 mean of the cycle divided by
the "Reference Diploid" value. For all cycles except Diploid, the
typical "DI" is also still reported.
A new function has been added to the equation processor. The "F"
function can be used to convert a keyword value into a
floating-point number. This allows the keyword to be used in
calculations. The syntax is: F[expression], where expression is
typically a keyword. For example, the $P1B keyword stores the number
of bits used to store a value for parameter 1 in an FCS file.
New internal keywords have been added to provide access to the
External Reference Mean [ExtRefMean], and the Diploid-to-Standard
Ratio [DipToStdRatio].
The SetProperty DDE/OLE command has been enhanced for the
ExternalReference property.
A new ploidy index of -1 has been added for hypo-diploid models.
Ploidy index can be displayed using the MF_PLOIDYINDEX keyword.
Bug fix: Program crashed when S-Phase component was changed to a
single Gaussian. This has been corrected.
Bug fix: The Mac version did not properly list files on some
network drives. When the network drive was selected, files in the
application folder were displayed. Corrected.
Version 3.1 Service Pack 2
This service pack includes all of the corrections and
enhancements of version 3.1. It can be applied to a ModFit LT 3.0 or
ModFit LT 3.1 installation.
The Proliferation Wizard now detects conditions where there is
little or no Parent generation. The wizard locks the SD for the
Parent to the last known good SD and alerts the operator. This
addresses problems that could occur in analyzing a sequence of
proliferation data files where the Parent generation is not present
as the experiment progresses.
The X-zoom graphic is now placed behind the main graph by default,
instead of on top of it. It can be moved to be on top of the main
graph by selecting the main graph, choosing the Report->Send to back
command, and then selecting and moving the zoom graph.
Implemented "battery" usage system.
Bug fix: Corrected crash that could occur when an older version of
the proliferation model was opened in a report and used for analysis
in a newer version of the program.
Version 3.1 Service Pack 1
This service pack includes all of the corrections and
enhancements of version 3.1. It can be applied to a ModFit LT 3.0 or
ModFit LT 3.1 installation.
The program now supports 3 gates and displays gated data in the
second and third gates. See the Listmode appendix for more details.
Prolif wizard now supports export of "partition" information.
Options for export appear on the Other tab of the prolif wizard
dialog. A new tutorial describes how this feature can be used with
WinList to provide enhanced proliferation analysis.
The Proliferation wizard now supports a "floating" model option for
the daughter generations. On the Generations tab, you can choose
between the "Standard" and "Floating" model options. In the standard
model, the mean of each generation is dependent on the previous
generation, based on the spacing value. In the floating model, a
range is associated with each generation and the mean is allowed to
'float' in the model. This option should ONLY be used with
well-defined, visibly-separated generations.
Added a Lock SD option to the Parent Tab of the Proliferation
Wizard. This option is useful for proliferating samples in which the
parent population becomes indistinct. When the option is enabled,
the SD for the parent population can be edited. All generations use
the same SD as the parent, regardless of the state of the Lock SD
option.
The status bar now displays low and high X and Y values as gate
regions move or resize to allow for more precise positioning. You
can also display the position of a region by clicking and holding on
the region without moving it.
When the program opens, it is now positioned and sized to the
location and size which were last used.
The registration dialog now displays a confirmation dialog when a
valid unlock code is entered.
The User Login name is now stored in a keyword, MF_USERLOGIN, which
can be displayed on reports and stored in the database.
The program now uses the Unbiased Summation method for reducing
histogram resolution to 256 channels. This will likely improve RCS
values for histograms that have native resolution greater than
256-channels. It may also result in slightly different locations for
peaks found by the Peak Finder.
After selecting a batch of data files or reports, the program now
automatically displays the Edit Properties for Batch dialog to allow
batch properties to be adjusted prior to opening the first file.
Options and Configuration now provides separate options for
auto-scaling X and Y axes to allow greater control.
Bug fix: User edits to the X axis label are now left in place when
new data files are opened. If first characters of the label are the
default label "Channels", the program will auto-label the axis.
Otherwise, the user label is left alone.
Bug fix: Graphics now update correctly for first-time DDE connection
with Winlist on PC.
Bug fix: The program could crash if gating resulted in zero events
in the histogram. The crash would occur when trying to fit the
histogram. This condition could also occur with DDE connections to
WinList.
Bug fix: The Proliferation Wizard did not reposition the parent
range if the parent position was adjusted in the dialog when editing
the model. This led to cases where the model would fail to fit some
files if the parent population moved beyond the scope of the range.
Bug fix: program could crash if low or high channels for zoom
graphics were greater than 256 channels AND preference settings for
Desired Resolution were 256. Crash would occur when a new data file
was read into the report.
Bug fix: program could crash with file or report paths greater than
120 characters. Revisions now support paths to 250 characters.
Bug fix: When batch analyzing files using the "Fit with current
model" option, the program would slow down as it progressed through
the batch. An internal array was not properly cleared in this
scenario.
Bug fix: With auto-scaling disabled, user-defined scale didn't apply
when advancing to next batch file while in scale view. This has been
corrected.
Version 3.1
Several new keywords have been documented.
The Edit Keywords dialog now uses an Add button instead of a Copy
button. The added equation will have a standard default value.
OLE features have been enhanced to provide more extensive OLE
Automation capabilities.
A Reset button has been added to the batch controls.
A new AutoAnalysis setting has been added to make all G2M's
dependent. By default, this setting is enabled. This is a
SIGNIFICANT CHANGE from previous versions that greatly improves
operator-to-operator consistency.
The logic that detects shifting of positions of internal standards
has been improved.
Crashes that could occur if AutoAnalysis could not create any cell
cycles in the model have been corrected.
Options and Configuration now has an Overrides section that can
apply settings to models in reports as they are loaded.
Models now have the ability to ignore internal standards in the
generation of debris and aggregate components. By default, internal
standards are now ignored by these components.
The Full Model Editor now displays the component ID when showing the
list of components in the model.
Bug fix: Sync and Prolif wizards didn't clean out analysis keywords
from database or keyword objects. Corrected 30Jul2001 (vi 969)
Bug fix: Debris component could report negative percentages and
areas in unusual cases. Corrected 30Jul2001. (vi 999)
Bug fix: The program did not read INCLUDE equation files from the
model's path if a model was opened in the Full Model Editor.
Corrected 26Jul2001.
Bug fix: A crash occurred when a model component (other than the
last component) was deleted from a model with the Full Model Editor.
Corrected 25Jul2001 (vi 1010)
Bug fix: A crash in peak finder could occur when a histogram had no
events. This unusual case could occur in a DDE connection with
WinList when the histogram in WinList is gated and shows no events.
Corrected 25Apr2001
Bug fix: The program can now identify hypo-diploid conditions where
the hypo-diploid peak is at a lower channel than an internal
standard.
Bug fix: The program now defeats S-phase cutoff warning when in OLE
automation mode. Corrected 4Sep2001 (vi 294)
Bug fix: A custom model in saved report did not "fit" when the
report was re-opened. Corrected 4Sep2001 (vi 975)
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