New Features
WinList 6.0 is a major version update with many new features and
enhancements.
Improved User Interface
Drag-and-drop support has been added throughout the program. Files can be
dropped onto the application to automatically open them for analysis. Histograms
and results can be dragged and dropped onto report windows to quickly add them
to report layouts. See
Drag and Drop
for additional details.
Intuitive cascade gating of histograms makes it incredibly quick to create
Boolean gates without having to type gate expressions. See
Cascade Gating and Boolean Gates for details.
Convenient toolbars for histograms and data sources provide quick access to the
most common tools and commands. Select region types, assign gates, add and
manage analysis pages, and much more. See
Histogram
Toolbar for details.
Reporting and Alerts
A full, page-layout reporting system has been added to the program. Multi-page
reports can be created with live links to analysis elements such as histograms
and result window. It is quick and easy to add keywords, gate legends, bitmaps,
logos, and of course free-form text. Report windows can be exported to PDF
format for convenient archiving and information sharing. See Report Windows
and Creating
Reports for more.
WinList now provides an Alert system that allows users to define alert
conditions based on region statistics. Each alert provides a user-defined name,
an alert condition, and a keyword that stores the alert. Alert conditions are
based on a user-selected region statistic, a mathematical test (=, <>, <, <=, >,
>=), and a target value for the alert. Alert objects can be added to reports,
displaying one color when the alert triggers, and a different color when it does
not. See Edit
Alerts for details.
Histograms, Regions, and Gates
Improvements have been made in how histograms are created and edited. The
Create Histograms dialog replaces the old Multiple Histogram Selection
dialog to provide a highly efficient means for creating many histograms at once.
In a similar way, the
Edit
Histogram dialog has been redesigned with a clean new look. Changing the
parameters displayed in a histogram is a simple matter of clicking on the
parameter label in the histogram and selecting a new parameter to view.
With the
histogram toolbar, you can draw any type of region by selecting the tool,
clicking and dragging on the histogram. Contour regions can now be previewed by
holding the mouse button down and moving the mouse, and you can use arrow keys
on the keyboard to move the active region. Regions now have a new option for
auto-positioning based on a user-defined percentile X and Y. The NStat region
can now auto-position based on this method.
Data Sources
Data source windows provide much more functionality than in previous versions.
Histograms can be "drawn" directly onto the data source, and a convenient grid
makes them easy to move, size, and align. Any number of pages can be added to a
data source to manage the number of histograms viewed at once, and moving
histograms from one page to another is a simple context menu action. See
Data Source
Windows for details.
A new option, Parameter Names, has been added to Data Source preferences. This
option allows you to choose the way that the program determines the names of
parameters in your listmode files. The default is "Automatic", which uses the
cytometer information to determine the appropriate keywords to use. You can also
choose to name parameters based on $PnS, $PnN, or a combination of these FCS
keywords.
Protocol Bundles
Protocols can now be saved in a Zip-compatible file, the
Protocol
Bundle format. This provides a simple and effective means for sharing
protocols with others. The Protocol Bundle contains all of the files that
WinList normally generates using the Save Protocol command, along with the FCS
files used by the protocol.
N-Color Compensation™
WinList now supports automatic compensation of BD Biosciences Diva files and
Beckman-Coulter FC500 files containing two datasets. Several user-preferences
determine how and when this feature is applied. See
Data
Source Preferences for details.
The Compensation Toolbox has a new button, "Create
Missing Histograms", which can be used to create bivariate histograms for
parameters enabled for compensation. When the button is clicked, the system
scans to see if any bivariates involving compensation parameters are not
currently shown. A dialog box with a list of the missing histogram is displayed,
allowing the user to choose which histograms to create. This feature makes it
very easy to create all of the 2P histograms that involved compensation
parameters. The data source toolbar also provides an option to create Scatter
Plot Matrix, "SPLOM
", of histograms that display all the combinations of parameters involved in
compensation.
We've improved the compensation system's auto-positioning routines that
automatically determine the best positions for the trace lines, resulting in
more accurate compensation settings.
iCyt Acquisition Plug-in
WinList is now capable of doing
real-time
acquisition and sorting with Reflection ™ cell sorting instruments from iCyt
Mission Technology. These instruments are designed for highly-automated parallel
sorting and are capable of very high throughput. Since the connection between
WinList and the Reflection is over TCP/IP, you can monitor experiments and
acquire data on any computer that has a internet connection.
Other Enhancements and Corrections
The histogram context menu now includes a Copy Data command. This command copies
the histogram's data in text format to the clipboard, where it can be easily
pasted into other applications.
The Copy Special dialog has been enhanced to allow selection of a target size
and DPI for histogram graphics. This makes it much easier to generate
high-quality histogram graphics for publications.
Corrected reading of some versions DakoCytomation Summit data files.
Results windows can now show statistic for "% Selected Gate". Choose a gate from
a list of the active gates, and each region in the results window will show its
events as a percentage of that gate. This option is useful to determine a
region's percentage of an ancestor gate, or of any gate in the data source.
A new wizard has been added to make it easy to add statistics in a Results
window to a database of results. The Results Database Wizard can be accessed by
right-clicking on a results window, or from the Options menu.
The Window menu has several changes and enhancements. The Arrange All submenu
has been renamed to Data Sources to better reflect the windows that are
affected. Added to this submenu are the names of all Data Source windows, making
it easy to activate a data source when more than one is open at a time. A new
Report submenu has been added to the Windows menu. This submenu has options to
Show All or Hide All reports. In addition, it shows a list of all open reports
so that a report can be easily activated.
Protocols now load faster because results window statistics are not updated
until the end of the protocol.
The Create Splom dialog now allows the selection of a gate to apply to the new
histograms.
Macros can now be up to 7000 lines long. Memory requirements on the Macintosh
version have been adjusted to accomodate this change.
Batch processing now provides options to save and print reports.
Two new region preferences have been added: "Show resizing handles" and
"Double-click action".
New statistics are available to display and database median values in ADC units.
A new region keyword has been added the contains the name of the gate that is
applied to the histogram that contains the region. To extract the gate name, use
the [R(id,stat)] keyword, and use GATENAME or GTN for the stat.
The program has a patch for Beckman Coulter FC 500 files to copy some of the
keywords missing in dataset 2 from dataset 1.
Bug fix: The CC result could produce a error value for regions containing a
single event.
Bug fix: Corrected problem with using Create Region Array to create FCOM regions
for an FCOM that uses more than 4 gates.
Bug fix: In WinList 5 Service Pack 4, macros that used the [FINISHBATCH] keyword to loop
through batch files could loop endlessly in some circumstances. This has been
corrected.
Bug fix: corrected decoding of FILEROOT keyword during batch processing.
Bug fix: Revise batch processing logic so that the first batch file(s) is
correctly opened when the Next Batch Item menu command is selected, or when a
button on the batch toolbar is pressed.
Bug fix: Corrected problem with compensation routines for linear vs. log
parameter compensation. In some cases, especially with high-resolution data,
WinList's trace lines computed an incorrect slope when compensating linear vs.
log parameters.
Bug fix: When reading some FCS3 listmode files, WinList could fail to find and
display the data portion.
Bug fix: When reading floating point listmode files, events with the highest
resolution value were sometimes incorrectly set to 0 during conversion to log.
Bug fix: The HyperLog transformation did not correctly handle Beckman-Coulter
32-bit data when displayed at "computed decades" (~6.01).
Bug fix: If a protocol was saved containing data source(s) with IDs not starting
with 1, the protocol did not replay properly. New protocols correctly handle
this problem. To edit existing protocols that show this problem, contact
technical support.
Bug fix: If saving a data source produced in a file that was larger than
99,999,999 bytes, the resulting output file was invalid due to restrictions in
the FCS 2.0 spec. The program now saves data sources in FCS3 format which
supports very large file sizes.
Bug fix: The program can now support reading FCS keywords of any length. This
corrects a problem in the ability to interpret the SPILL compensation keyword
for some Diva files. Some restrictions still apply to the length of keywords for
display purposes.
Bug fix: Contours of low-frequency populations in histograms that also have
high-frequency populations did not always display properly. Also, in some cases
the contour display showed unexpected line segments crossing contours. Both of
these issues have been corrected.
Bug fix: On fast PCs, remote scripts could sometimes appear to hang the program.
This can be corrected by specifying a timeout value for DDE communications.
Contact tech support for details.
Bug fix: When batch processing and printing files on the Mac, the Print Options
dialog was inadvertently displayed. This dialog is now suppressed during batch
processing and macro playback.
Bug fix: Print Screen on the Mac could fail in with some monitor configurations.
This has been corrected.
Bug fix: If no histograms were displayed and the Edit button on the Compensation
Toolbox was clicked, the program could crash trying to compute digital error
values.
2P region resizing handles now encompass only the region points; they do not
include the region's label.
1P range, 2P rectangle, and 2P ellipse regions now begin drawing as soon as the
mouse button is pressed. This change is a result of the use of the new region
toolbar, making the drawing operation more efficient.
In Color Event mode, 1P histograms now show a polygon for the total histogram
data in addition to the cluster polygons.
The SED histogram subtraction algorithm contained a bug that could affect the
reported % positives.
The Close All Windows command now reinitializes the gates and regions to their
default states.
The Gate dialog and Region List dialog are now closed automatically by the Close
All Windows command. This corrects a crash that occurred if either dialog was
open and a protocol was loaded.
Corrected parameter range checking logic for data stored floating-point format.
Previous versions could crash if a floating-point parameter value exceeded the
resolution specified in $PnR.
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